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Juan de Grijalva (born around 1489 in Cuéllar, Crown of Castille - 21 January 1527 in Nicaragua) was a Spanish conquistador, and relation of Diego Velázquez. He went to Hispaniola in 1508 and to Cuba in 1511.

Grijalva was one of the earliest to explore the shores of Mexico. According to Hernán Cortés, 170 people went with him, but according to Pedro Mártir, there were 300 people. The main pilot was Antón de Alaminos, the other pilots were Juan Álvarez (also known as el Manquillo), Pedro Camacho de Triana, and Grijalva. Other members included Francisco de Montejo, Pedro de Alvarado, Juan Díaz, Francisco Peñalosa, Alonso de Ávila, Alonso Hernández, Julianillo, Melchorejo, and Antonio Villafaña. They embarked the port of Matanzas, Cuba with four ships in April 1518.